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. 2021 Apr 6;10(4):819. doi: 10.3390/cells10040819

Figure 3.

Figure 3

Circulating extracellular vesicles (EVs) contain a majority of the ccf-DNA that has mitochondrial origin. (A) PicoGreen quantification of ccf-DNA in serum of healthy volunteers (n = 4) and subjects with lung adenocarcinoma (n = 12) admitted to UTMB’s clinic. (B) Serum contain active DNase/s. Changes in the fluorescent units of Λ DNA incubated with increasing volume of serum (40 and 80 μL). (C) Ccf-DNA is DNase insensitive. Serum samples of three lung adenocarcinoma subjects were incubated with recombinant DNase and changes in DNA concentration measured with PicoGreen™ expressed in fluorescent units are shown. Ccf-DNA is localized within lumen of EVs. Comparison in the amount of DNA between input, serum after EVs isolation (supernatant) and isolated EVs (pellet) of three subjects using (D) mtCOXIII and (E) nuACTB primers.