Figure 4.

PCR products amplified with primers annealing to the distal untranslated exon 1 and CDS. A clearly visible band was found in all testis samples, a weak band only in hypothalamus of female #1. Annealing positions of corresponding forward and reverse primers in the distal untranslated exon 1 and the CDS of CYP19A3 are shown in the inserted Figure 1. this transcript variant was detected but represented by a considerably weaker band. These transcripts were also present in both peripubertal ovarian samples based upon sequencing of isolated PCR products (not shown). Direct sequencing analysis of PCR products and the presence of only one band in the gel clearly indicated that a sequence derived from distal untranslated exon 2 was not spliced into any of these products.