Table 3.
Primers used for RT-PCR.
| Name | Sequence | Length (nt) | Pos in NC_010443.5 | Orient. | |
|---|---|---|---|---|---|
| DistUTR1for1 | TGGCTTTCTCTCCCTCTCCA | 20 | 120367673 | 120367692 | for |
| DistUTR1for2 | ACATCAAGCGGTTAGGGTTCA | 21 | 120367836 | 120367856 | for |
| GLDNfor | AGCACATCCGCACAGAGAG | 19 | 120398553 | 120398571 | rev |
| GLDNrev | GCCAGGGCAGCCTTTATATG | 20 | 120398934 | 120398915 | rev |
| DistUTR2for1 | CCGCGCATCATTAGCAAAACT | 21 | 120477287 | 120477307 | for |
| DistUTR2rev2 | GCGGCTGTTTAAGAACCGGT | 20 | 120477290 | 120477271 | rev |
| DistUTR2for2 | CATCATTAGCAAAACTCACCAT | 22 | 120477292 | 120477313 | for |
| DistUTR2rev1 | ATGCGCGGCTGTTTAAGAAC | 20 | 120477294 | 120477275 | rev |
| ProxUTRfor | CAAATATGTCTTGTCTAAGTGTCCA | 25 | 120525069 | 120525093 | for |
| CDSrev | TTGCAATGCTGCCAAAAAGGA | 21 | 120525325 | 120525305 | rev |
| CYP19A3_for | CCTCTGGAAAGCTGTTCGACCTTTC | 25 | 120541804 | 120541828 | for |
| CYP19A1_for | CCTCTGGAAAGCTGTTAGAACTTAT | 25 | 120611584 | 120611608 | for |
| CYP19A2_for | CCTCTGGAAAGCCGTTAGAACTTAC | 25 | 120674035 | 120674059 | for |
| CYP19rev | GTAGCCCAAGTCATTGCGG | 19 | 120677180 | 120677162 | rev |