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. 2021 Apr 7;9(4):770. doi: 10.3390/microorganisms9040770

Figure 3.

Figure 3

Vaccination with S. Typhi porins induces functional central memory CD4+ and CD8+ T cell responses. C57BL/6J mice were injected intradermally in the ear with vehicle (Veh), proteinase K-digested porins (PorK), porins (PorID), or intraperitoneally with porins (PorIP) or heat-inactivated S. Typhi (HIS) at day 0. All groups were boosted at day 14 with porins injected intradermally in the ear. T cells from the skin dLN were analyzed at day 28. Representative plot of the count of cell number (A) for both CD4+ (B) and CD8+ (C) populations in the skin dLN. Representative plot used (D) to analyze expression of IFN-γ and IL-17 in CD4+ and CD8+ dLN T cells following stimulation with S. Typhi porin peptides. Total number of IFN-γ- (E) and IL-17- (F) producing CD4+ T cells. Total number of IFN-γ- (G) and IL-17- (H) producing CD8+ T cells. Central memory phenotype (CD44+, CD62L+) was evaluated in skin dLN porin-specific T cells. Representative plot (I) used to analyze central memory CD4+ (J) and CD8+ (K) populations (CM, central memory, EM, effector memory, N, naive) (n = 7; 2 independent experiments; significant difference for a Kruskal–Wallis test ** p < 0.05 with Dunn’s multiple comparison).