Figure 1.

Generation of V-set and immunoglobulin domain-containing 1 (VSIG1)^−/− mice using clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 (CRISPR/Cas9) system. (a) Mouse VSIG1 contains eight exons (shaded boxes) interrupted by three introns. The ATG translation initiation codon is located in the second exon. Genomic sequence of the CRISPR target site in VSIG1^+/+ and VSIG1^−/−. Letters in the box indicate the target mutation. (b) Genomic DNA PCR from KO mice. Arrowhead means wild-type (WT) band, while arrow means VSIG1 knock out (KO) of male and female. (c) Comparison of peptide sequences between wild-type (WT) and knock out (KO) VSIG1 mice. Amino acids identical between two sequences are shown asterisks. # means stop cordon.