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In vitro fertilization (IVF) assay using VSIG1 knockout (KO) mouse sperm. (a) The fertilization ratio of intact eggs inseminated with capacitated cauda epididymal sperm from VSIG1 KO and wild-type (WT) male mice. (b) Cumulus-free eggs were inseminated with capacitated cauda epididymal sperm derived from WT and VSIG1 KO mice, and the fertilization ratio was determined.
