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Number of primer sequences for genomic PCR (polymerase chain reaction) and RT-PCR (reverse transcription PCR).
| Sense Primer (5′to 3′) | Anti Sense Primer (5′to 3′) | |
|---|---|---|
| VSIG1 gPCR | P1: TACTACTCTGAAGGTGGACAG | P2: GTTTGACTAAGACAGTGACGAC |
| VSIG1 rtPCR | P3: TATTGCATATGCAGCCAGCAGAC P5: GGCTACTAATCCCGGTAATGCAT |
P4: GTACACTGGTAACCTTGTTC |
| GAPDH rtPCR | AGATTGTCAGCAATGCATCCTG | TGCTTCACCACCTTCTTGATGT |
gPCR; genomic polymerase chain reaction, rtPCR; reverse transcription polymerase chain reaction.
