Figure 3.

Cold-inducible RNA-binding protein (CIRP) knockout decreases leukocyte infiltration in ischemic tissue. (a) The scatter plot displays the relative amount of CD45+ (pan-leukocyte marker) cells (per mm²) of ischemic gastrocnemius muscles of wildtype and CIRP -/- mice 7 days after femoral artery ligation (FAL). Data are means ± S.E.M., n = 5 per group. * p < 0.05 (CIRP -/- vs. control) by unpaired, two-sided student’s t-test. (b) Representative immunofluorescence stainings of ischemic gastrocnemius muscles of CIRP wildtype control (left) and CIRP -/- (right) mice 7 days after FAL. Smaller images display single channels, large images merged channels of leukocytes (anti-CD45, green) and nucleic acid (DAPI, blue) stains. Scale bars 50 µm.