Table 3.
Assignment, retention times, UV and MS spectral data of phenolic compounds in methanol extracts of Hyssopus officinalis subsp. aristatus. a Identification by comparing with commercial reference compounds. b Tentative identification by comparing acquired UV and MS spectral data with literature data.
| Peak No. | tr (min) | UV λmax (nm) | ESI-MS Data (m/z) | Assignment |
|---|---|---|---|---|
| 1 | 4.255 | 280 | 395.1 [2M-H]−, 197 [M-H]−, 153.1 | Syringic acid b |
| 2 | 9.107 | 218, 240, 298 sh, 326 | 707.1 [2M-H]−, 353.1 [M-H]−, 191 | Chlorogenic acid (5-O-caffeoylquinic acid) a |
| 3 | 10.954 | 218, 238, 298 sh, 328 | 623.1 [2M-H]−, 311.1 [M-H]−, 134.1 | Caffeoyl pentoside b |
| 4 | 12.087 | 218, 238, 296 sh, 326 | 735.2 [2M-H]−, 367.1 [M-H]−, 173.1 | Feruloylquinic acid b |
| 5 | 15.339 | 256, 266 sh, 356 | 463.1 [M-H]−, 300.1 | Quercetin O-hexoside b |
| 6 | 17.215 | 252, 266, 348 | 607.2 [M-H]−, 299.1, 284 | Diosmetin O-deoxyhexosyl-hexoside b |
| 7 | 18.461 | 286 sh, 328 | 719.1 [2M-H]−, 359 [M-H]−, 197, 161.1 | Rosmarinic acid a |