Table 2.
In vitro activity of compounds 4a–l and 5a–d (200 μg/8 mm disc); the broad-spectrum antibacterial drugs Gentamicin sulfate, Ampicillin trihydrate, and the antifungal drug Clotrimazole (100 μg/8 mm disc) against Staphylococcus aureus American type culture collection (ATCC) 6571 (SA), Bacillus subtilis ATCC 5256 (BS), Micrococcus luteus ATCC 27,141 (ML), Escherichia coli ATCC 8726 (EC), Pseudomonas aeruginosa ATCC 27,853 (PA), and the yeast-like pathogenic fungus Candida albicans MTCC 227 (CA).
| Comp. No. |
C log Pc | Diameter of Growth Inhibition Zone (mm) a | |||||
|---|---|---|---|---|---|---|---|
| SA | BS | ML | EC | PA | CA | ||
| 4a | 3.701 | 14 | 13 | 15 | - | - | 10 |
| 4b | 4.146 | 15 | 16 | 17 | - | - | 12 |
| 4c | 4.716 | 14 | 17 | 13 | - | - | - |
| 4d | 4.716 | 15 | 13 | 14 | - | - | - |
| 4e | 4.242 | 12 | 11 | 10 | - | - | 13 |
| 4f | 4.092 | 11 | 12 | - | - | - | 12 |
| 4g | 4.092 | 13 | 12 | 11 | - | - | 16 |
| 4h | 5.113 | 15 | 12 | - | - | - | - |
| 4i | 5.113 | 16 | 12 | - | - | - | - |
| 4j | 4.395 | 19 (4) b | 12 | 16 | 12 | 13 | 11 |
| 4k | 4.395 | 17 | 14 | 15 | 14 | - | 12 |
| 4l | 5.535 | 19 (4) b | 21 (2) b | 18 (16) b | 15 | 12 | 11 |
| 5a | 3.789 | 23 (1) b | 26 (1) b | 21 (1) b | 14 | 13 | - |
| 5b | 4.103 | 20 (8) b | 23 (1) b | 20 (1) b | 16 | 17 | - |
| 5c | 4.712 | 26 (1) b | 30 (0.5) b | 22 (1) b | 19 (4) b | 18 (4) b | - |
| 5d | 5.595 | 28 (1) b | 29 (1) b | 26 (1) b | 22 (1) b | 20 (2) b | - |
| Gentamicin sulfate | 27 (1) b | 26 (2) b | 20 (2) b | 22 (0.5) b | 21 (0.5) b | NT | |
| Ampicillin trihydrate | 22 (2) b | 23 (1) b | 20 (2) b | 16 (8) b | 16 (8) b | NT | |
| Clotrimazole | NT | NT | NT | NT | NT | 21 (4) b | |
a (-): inactive (inhibition zone < 10 mm), b Figures shown in parentheses represent the minimal inhibitory concentration (MIC) values (μg/mL), c Calculated using the CS ChemOffice Ultra version 8.0, CambridgeSoft, Cambridge, MA, USA), NT: not tested. SA, Staphylococcus aureus; BS, Bacillus subtilis; ML, Micrococcus luteus; EC, Escherichia coli; PA, Pseudomonas aeruginosa; CA, Candida albicans. High activity (>18 mm) values have been bolded for emphasis.