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. 2021 Apr 7;22(8):3810. doi: 10.3390/ijms22083810

Figure 1.

Figure 1

Generation of an Nme7 knock-out Sprague Dawley rat model. (A) At the top, part of the sequence of Nme7, coding exon 4, is shown together with the locations of two sets of guide (g)RNAs. The bottom part of the figure shows the corresponding amino acid sequence (standard one-letter code), indicating its change after the induced deletion. (B) Ratio of the genotypes of surviving SDNme7 rats at three distinct time points (embryo: E13.5–E15.5, newborn: P1, and weanling: P28). The red dashed line corresponds to the expected Mendelian ratio of 25% (SDNme7+/+—grey bars):50% (SDNme7+/−—striped bars):25% (SDNme7−/−—black bars). (C) Western blot showing the lack of Nme7 protein in SDNme7−/− rat testes. Stripped membrane was used for γ-tubulin protein detection.