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. 2021 Apr 7;22(8):3819. doi: 10.3390/ijms22083819

Figure 1.

Figure 1

Expression of genes encoding E1-S transporters in model cell lines. (a) Expression of 14 genes involved in E1-S transport included in the PCR arrays for human drug transporters (PAHS-070Z) were examined in model cell lines, Ishikawa and HEC-1-A, and in control cell line, HIEEC. The data were normalized using normalization factor based on the expression of two most stably expressed genes (ACTB and HPRT1). (b) The expression of 15 ‘E1-S transporter genes’ was investigated in three independent experiments with two to three replicates, using newly designed primers. Statistical analysis was performed using Kruskal–Wallis test with Dunn’s multiple comparison test correction. (c) Based on normalized mRNA data, fold regulation (FR) was calculated for each gene in Ishikawa and HEC-1-A versus HIEEC. (d) Venn diagram representation of genes with significant difference in expression in Ishikawa and/or HEC-1-A compared to HIEEC. FR—fold regulation, ns—p-value > 0.05, * p-value ≤ 0.05, ** p-value ≤ 0.01, *** p-value ≤ 0.001, **** p-value ≤ 0.0001. Underlined genes are involved in E1-S efflux from cells. Data are shown as mean ± SD.