Table 2.
Data on the antibodies used for Western blotting or immunohistochemistry (IHC).
| Antigen, Type of Antibodies | Manufacturer, Catalogue ID, Batch ID, Name of the Source |
Antigen Sequence | Species Raised, Monoclonal, Polyclonal, Antigen Purified |
Positive and Negative Controls |
Method Where Antibodies Were Used |
|---|---|---|---|---|---|
| anti-STS | Provided by Dr. Schuler [37,38]. | whole protein | rabbit, polyclonal |
HepG2 (positive), MCF-7 (negative) | Western blot |
| anti-OSTβ rabbit, polyclonal |
HPA008533, lot A105958; Sigma Aldrich, St. Louis, MO, USA |
VLHLDEAKDHNSLNNLRETLLSEKPNLAQVELELKERDVLSVFLPDV | rabbit, polyclonal, affinity purified |
colon (positive) liver (negative) |
IHC |
| anti-ABCG2 | ab3380, lot GR308999-5, clone number BXP-21; Abcam, Cambridge, UK | 126 amino acids, 271–396 GeneBank accession #AF098951] | mouse monoclonal | colon (positive) liver (negative) [39] |
IHC |
| anti-OATP1B3 | HPA004943, lot E104368; Sigma Aldrich, St. Louis, MO, USA |
QGKDTKASDNERKVMDEANLEFLNNGEHFVPSAGTDSKTCNLDMQDNAAAG | rabbit, polyclonal, affinity purified |
WB: HEPG2 (positive) MCF7 (negative) IHC: liver tissue (positive), cardiac muscle (negative) |
IHC |