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. 2021 Apr 7;22(8):3834. doi: 10.3390/ijms22083834

Figure 4.

Figure 4

Non-Exact repair outcomes in reactions containing no repair ssODN. Gene repair reactions were performed using the in vitro system in the absence of and ssODN repair template, to determine whether or not the secondary products seen in Figure 3 were influenced by the presence of a repair template. Unedited sequences are displayed in gray and edited sequences are shown in dark orange. (A) Indel products seen when targeting the 1364 LacZ site. (B) Indel products seen when targeting the 1344 LacZ site. (C) Indel products seen when targeting the 1228 LacZ site. (D) Specific resultant repair outcomes seen in the three non-ssODN reactions, deconvoluted and aligned using DECODR [61]. The alignment of the gRNA is shown as a green bar at the top of the reference sequence (with a red end for the PAM site), and the specific cut site on the S-strand of the DNA is shown as a pipe (|). Deleted bases are represented by dashes.