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. 2021 Apr 7;22(8):3829. doi: 10.3390/ijms22083829

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Brain tissue stained with Prussian blue (Black arrows). (A) Control group showing a negative Prussian blue stain. (B) IONPs group showing blue spots representing the iron contents by Prussian blue stain. (C) IONPs + Q25 group showing moderate blue spots representing the iron contents by Prussian blue stain. (D) IONPs + Q50 group showing a few blue spots representing the iron contents by Prussian blue stain. (E) IONPs + Q100 group showing very few dispersed blue spots representing the iron contents by Prussian blue stain. ×400. Scale bar = 20 µm.