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. 2021 Apr 7;22(8):3829. doi: 10.3390/ijms22083829

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunohistochemistry of Bcl2 in brain tissue. (A) Control group showing negative (black arrow) and strong (red arrow) Bcl2 positive brown immunostained neurons. (B) IONPs group showing negative (black arrows) and a few strong (red arrows) and weak (green arrow) Bcl2 positive brown immunostained neurons. (C) IONPs + Q25 group showing negative (black arrows) Bcl2 brown immunostained neurons. (D) IONPs + Q50 group showing moderate (blue arrows) and weak (green arrows) Bcl2 positive brown immunostained neurons. (E) IONPs + Q100 group showing strong (red arrows) Bcl2 positive brown immunostained neurons. ×400. Scale bar = 20 µm.