Figure 4.

Cre recombinase expression in glial fibrillary acidic protein (GFAP)⁺ astrocytes but not neuronal nuclei (NeuN)⁺ neurons after infected by adeno-associated virus serotype-9 (AAV9) GFAP::Cre in the mouse cortex.

(A) Representative high-resolution confocal images showing colocalization of Cre recombinase with GFAP signals at 14 days post viral injection (14 dpi). AAV9 hGFAP::Cre at 1 × 10¹⁰ gc/mL + FLEX-CAG::mCherry at 1 × 10¹² gc/mL, 1 μL in mouse cortex. (B) Representative high-resolution confocal images showing that Cre recombinase was not colocalized with NeuN signals (14 dpi). Scale bars: 20 μm.