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. 2021 Apr 7;9(4):357. doi: 10.3390/vaccines9040357

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Recombinant TgRPP2 induces the apoptosis of murine Ana-1 cells. Ana-1 cells were preincubated with different concentrations of rTgRPP2 for 48 h. Quadrant Q3 represents early-stage apoptosis (annexin V+/PI−), while quadrant Q2 represents late-stage apoptosis (annexin V+/PI+). Values were evaluated using one-way ANOVA analysis, followed by Dunnett’s test, and expressed as the mean ± standard deviation of three independent experiments (n = 3). * p < 0.05 and *** p < 0.001 compared with the blank group or the control group.