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Determination of total IgG (a) and subclass IgG1 (b) and IgG2a (c) antibodies in the sera from animals vaccinated with PBS, pET32a vector protein, and rTgRPP2. Results were evaluated using one-way ANOVA analysis, followed by Dunnett’s test, and shown as the mean of OD450 ± standard deviation (n = 5). ** p < 0.01 and *** p < 0.001 compared with the blank group or the control group.
