Table 2.
ITC Experimental Values | |||
---|---|---|---|
N of Sites | KITC (M−1) | ΔH (Kcal mol−1) | |
Mg2+ | 3 | 3.25 × 106 | −1.6 |
Ca2+ | 1 | 7.68 × 105 | −13.1 |
Zn2+ | 4 | 1.77 × 107 | −9.9 |
N of sites: number of protein binding sites for metal ions; KITC: binding constant or isothermal titration calorimetry association constant, ΔH: enthalpy related to KITC.