Figure 2.

Kinase activity of FN3K and the inactive mutant enzyme FN3K–K41L as assessed by the amount of phosphate released per well (nmol) using a 1-deoxy-1-morpholino-D-fructose (DMF) acceptor substrate at increasing enzymatic concentration levels (µg/mL). Data are depicted as single data points with their fitted regression line in the linear range and 95% confidence interval.