Figure A2.

Time-dependent changes (%) in autofluorescence (AF) values after treatment of a cataractous human lens suspension (different suspension as the one used in Section 4.7.2 “Kinetics of FN3K treatment”) with 20 µL of a solution containing fructosamine-3-kinase (FN3K, 125 µg/mL) and its cofactors ATP (12.5 mM) and MgCl₂ (5 mM) in PBS, (2) the cofactors solely and (3) a solution containing ADP solely (12.5 mM). The experiments were performed in duplicate using the same setup as described in Section 4.7.2. While clear decreases in AF values were observed for the FN3K enzyme, slight increases in AF values were noticed for the solutions containing ATP/MgCl₂ and ADP. Based on these results, we could conclude that neither the added ATP nor the ADP that was not released by the mutant enzyme was responsible for the observed decreases in autofluorescence after FN3K treatment in our experiments.