Table 1.
EV counts obtained for platelet-derived EVs by flow cytometry (Gallios vs. CytoFLEX LX) and by NTA (scatter mode vs. fluorescence mode after staining with CMO or LA-AF555).
| Flow Cytometry | |||||
|---|---|---|---|---|---|
| Device | Events/µL | LA+ EVs/µL | LA+ EVs [% of All Events in the EV Gate] |
LA+CD41+ EVs/µL | LA+CD41+ EVs [% of All Events in the EV Gate] |
| Gallios | 1.5 ± 0.5 × 106 | 1.4 ± 0.6 × 106 | 93 ± 4 | 1.3 ± 0.5 × 106 | 87 ± 6 |
| CytoFLEX LX | 6.0 ± 5.5 × 107 | 3.3 ± 2.5 × 107 | 64 ± 13 | 1.7 ± 0.9 × 107 | 40 ± 20 |
| Nanoparticle Tracking Analysis | |||||
| ZetaView PMX-110 |
Particles/µL
[Scatter Mode] |
LA+ Particles/µL
[Fluorescent Mode] |
LA+
[% of All Particles in Scatter] |
CMO+ Particles/µL
[Fluorescent Mode] |
CMO+
[% of All Particles in Scatter] |
| 4.0 ± 1.7 × 108 | 3.3 ± 1.7 × 107 | 9.5 ± 4 | 1.8 ± 0.9 × 108 | 36 ± 7 | |
n = 8; same EV batches used for all measurements.