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. 2021 Apr 8;13(4):636. doi: 10.3390/v13040636

Figure 2.

Figure 2

Electrophoretogram of amplicons from the CMNV RT-nPCR assay and phylogenetic analysis. (a) Electrophoretogram of amplicons from the second-step PCR of the CMNV RT-nPCR assay. M: DL2000 molecular weight marker. Lanes 1–8: the PCR products of four RNA samples from sea cucumbers’ intestines (each sample was done with two replicates; samples 1, 2, 3, and 4 were shown in lanes 1 and 2, 3 and 4, 5 and 6, 7 and 8, respectively). P: positive control; N: negative control. (b) Analysis of the phylogenetic tree based on the deduced amino acid sequences of the RdRp gene from the CMNV-positive sea cucumber sample and other nodaviruses (abbreviations of other viruses shown in Table 1). The CMNV isolate of the sea cucumber sampled from farming ponds is highlighted in red. Viral species of Alphanodavirus genus and Betanodavirus genus are shown in pink and lavender background, respectively. The phylogeny tree was derived using the neighbor-joining method by the MEGA 6.0 program. The scale bar is 0.01.