Table 3.

Studies using next-generation digital pathology for the quantification of blood vessels.

Cancer Type	Markers	Scanner/Microscope	Quantification System	References
Breast cancer	CD34	Olympus BX41 (Olympus, Tokyo, Japan)	Cell D software (Olympus, Tokyo, Japan)	[178]
Breast cancer	CD34	NanoZoomer (Hamamatsu Photonics, Hamamatsu City, Japan)	Slidepath Image Analysis system (Leica Biosystems Inc., Richmond, IL, USA)	[179]
Breast cancer	CD34	TissueFAXS (TissueGnostics, Vienna, Austria)	HistoQuest (TissueGnostics, Vienna, Austria)	[180]
Breast cancer metastasis	CD31	Panoramic 250 (3Dhistech, Budapest, Hungary)	Visiopharm image analysis software (Visiopharm, Copenhagen, Denmark)	[181]
CRC	CD31	Mirax slide scanner system (3Dhistech, Budapest, Hungary)	Image J software (NIH, Bethesda, MD, USA)	[182]
CRC	CD31	TissueFAXS (TissueGnostics, Vienna, Austria)	StrataQuest (TissueGnostics, Vienna, Austria)	[183]
ESCC	CD31	TissueFAXS (TissueGnostics, Vienna, Austria)	HistoQuest, TissueQuest (TissueGnostics, Vienna, Austria)	[184]
Human tumor	CD31, CD34	Aperio (Leica Biosystems Inc., Richmond, IL, USA)	Fiji, Image J software (NIH, Bethesda, MD, USA)	[185]
Melanoma	CD31	Aperio CS Scanner (Leica Biosystems Inc., Richmond, IL, USA)	Aperio image analysis system (Leica Biosystems Inc., Richmond, IL, USA)	[186]
Pancreatic cancer	CD31	n.s.	The Ariol™ image analysis system (Genetix, New Milton, England)	[187]
Renal cancer	CD34	Zeiss Axio Scan.Z1 (Zeiss, Jena, Germany)	Developer XD, Tissue Studio (Definiens, Munich, Germany)	[188]
Rectal cancer	CD34	ScanScope CS (Aperio Technologies, Vista, CA, USA)	ImageScope (Aperio Technologies, Vista, CA, USA)	[189]
Tongue cancer	PNAd	ScanScope T3 (Aperio Technologies, Vista, CA, USA)	Image J software (NIH, Bethesda, MD, USA)	[190]

CRC, colorectal cancer; n.s., not specified; ESCC, esophageal squamous cell carcinoma.