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. 2021 Apr 8;22(8):3852. doi: 10.3390/ijms22083852

Figure 2.

Figure 2

Human PH-Fibs do not exhibit reduced expression of miRNA processing genes; in addition, HDACi does not increase expression of miRNA processing genes in human PH-Fibs. (A) qRT-PCR analysis of the mRNA levels of miRNA processing genes including Dorsha, DGCR8, XPO5, Dicer and TARBP2 in human CO- (n = 4) and PH-Fibs (n = 4). (B) Western-blot analysis of Dicer and TARBP2 protein in human CO- (n = 4) and PH-Fibs (n = 4). (C) Quantification of protein levels of Dicer and TARBP2 based on WB results in Figure 2B and statistical analysis of ratios of Dicer/β-actin and TARBP2/β-actin in PH Fibs, compared to CO-Fibs. (D) qRT-PCR analysis of the mRNA levels of miRNA processing genes in human PH-Fibs (n = 4) treated with DMSO (control) or HDACi (SAHA and Apicidin) for 48 h. Data are presented as mean ±S.E.M, * p < 0.05, ** p < 0.01 PH-Fibs vs. CO-Fibs or PH-Fibs treated with HDACi vs. DMSO.