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. 2021 Apr 8;22(8):3852. doi: 10.3390/ijms22083852

Figure 4.

Figure 4

miR-124-1 gene in human PH-Fibs is more condensed (closed), compared to that in CO-Fibs, as assessed using DNase I sensitivity assay. (A) Schematic presentation of DNase I Sensitivity Assay. DNA in closed chromatin (a and c) is not accessible, thus resistant to DNase I digestion, so the ratios of these DNA in DNase treated genomic DNA pool versus in uncut genomic DNA pool will be close to 1 or above 1. However, DNA in more loosely packed chromatin (b and d) will be digested by DNase I, so the ratios of these DNA in DNase treated genomic DNA pool versus in uncut genomic DNA pool will be less than 1. (B) DNase I Sensitivity Assay was used to determine the chromatin structure of the miR-124-1 gene’s promoter (−110/−4), proximal promoter (−240/−160) and enhancers (−711/−590 and −1543/−1466) in CO- (n = 4) and PH-Fibs (n = 3). Data are presented as mean ±S.E.M, * p < 0.05 PH-Fibs vs. CO-Fibs.