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. 2021 Apr 8;22(8):3851. doi: 10.3390/ijms22083851

Figure 5.

Figure 5

In Vitro Analysis of MSC-EVs Activity on Migration of Cells Involved in Wound Healing Process and Vessel Formation. (A) Scratch test assay on different cell types (fibroblasts, keratinocytes and endothelial cells) for measuring the pro-migration effect of MSC-EVs. The wound closure was measured 24 h after treatment and expressed as percentage of the initial wound area; (B) representative micrographs and quantitative analysis of capillary-like structure formation test on endothelial cells treated for 24 h. Capillary-like structures length was measured and expressed as ratio in comparison to untreated cells (NT). Cells were treated with DMEM 0% FBS for untreated cells (NT), DMEM 0% FBS with 50,000 EVs/cell for BMSC or ADSC-EVs (BMSC or ADSC-EV) and different positive controls (CTR+) for each cell type (MCDB131 medium for endothelial cells, FGM-2 Growth Media for fibroblasts, and KGMTM Gold Keratinocyte Growth Medium for keratinocytes). For capillary-like structures formation test, MCDB131 medium was used as CTR1+ and 0.1 µg/mL EGF as CTR2+. Statistical analysis was performed comparing each sample with NT (*: p < 0.05, **: p < 0.01, ***: p < 0.005, ****: p < 0.001) or comparing BMSC-EV and ADSC EV ($: p < 0.05, $$: p < 0.01, ns: not statistically significant).