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. 2021 Apr 8;10(4):840. doi: 10.3390/cells10040840

Figure 4.

Figure 4

TNF modulates astrocytic differentiation through the classical Gfap marker. (A) Kinetic of Gfap mRNA gene expression obtained by RT-PCR. Each time point is normalized to its 6 h of differentiation. Results are given as mean ± SEM (n = 3) * p < 0.05 and ** p < 0.01. (B) Effect of TNF treatment on Gfap gene expression obtained by RT-PCR. Each time point is normalized to its FBS control (Ctrl = 1) ** p < 0.01. Results are given as mean ± SEM (n = 4). (C) Immunocytochemistry showing GFAP protein expression (red) in TNF treated cells at 6, 24, and 72 h. Nuclei were counterstained by DAPI (blue). Scale bar: 50 µm. (D) Immunoblots from TNF treated and untreated at 0, 24, and 72 h of differentiation. GFAP protein expression is affected by TNF, ** p < 0.01.