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. 2021 Apr 8;10(4):840. doi: 10.3390/cells10040840

Figure 5.

Figure 5

TNF increases neural progenitor cell proliferation. (A) Kinetic of mKi67 mRNA gene expression obtained by RT-PCR. Each time point is normalized to its 6 h of differentiation (Ctrl = 1). Results are given as mean ± SEM (n = 3) * p < 0.05, ** p < 0.01 and *** p < 0.001. (B) Effect of TNF treatment on mKi67 gene expression obtained by RT-PCR. Each time point is normalized to its FBS control (Ctrl = 1) * p < 0.05. Results are given as mean ± SEM (n = 4). (C) Immunocytochemistry showing KI67 protein expression (red) in TNF treated cells at 6, 24, and 72 h. Nuclei were counterstained by DAPI (blue). Scale bar: 50 µm. (D) TNFα induces the NSP reformation. After 6, 24, and 48 h of NSP-derived astrocyte differentiation treated with TNFα (50 ng/mL), an NSP reformation assay was performed in presence of EGF and FGF (20 ng/mL both, 10,000 cells/well). Ten days after, the number of reformed NSP at 6, 24, and 48 h was counted (n = 3, mean ± SEM).