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. 2021 Apr 8;13(8):1780. doi: 10.3390/cancers13081780

Figure 3.

Figure 3

Effect of direct 3 min cold atmospheric plasma (CAP) (COST jet) treatment, TMZ (40 µM for U251, and 72 µM for U87-MG and T98G) and their combination on human TMZ-sensitive (U251) and TMZ-resistant (U87-MG and T98G) GBM spheroids. (a) Schematic representation of the CAP treatment setup; (b–d) quantification of spheroid area: (b) total spheroid area, (c) area of the spheroid core, and (d) Cytotox green intensity (dead cell confluence) in U251, U87-MG and T98G spheroids. Statistical analysis was performed using one-way analysis of variance (ANOVA) with Tukey’s comparison analysis. The results are derived from three independent biological replicates and are shown as the mean ± standard error of the mean (SEM). Statistically significant differences were found between the untreated control and corresponding treated samples. * = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001, ns = not significant. (e) Representative images of U251, U87-MG and T98G spheroids following CAP treatment, in flowing convergence image: red represents live cells, green represents dead cells within the spheroid.