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. 2021 Apr 8;10(4):574. doi: 10.3390/antiox10040574

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Identification of MG-H1-modified proteins in the HIP from KO/VB6(−) mice. HIP homogenates of WT/VB6(+) mice or KO/VB6(−) mice were separated by two-dimensional gel electrophoresis. MG-H1-modified proteins were identified by Western blot analysis using anti-MG-H1 antibody. The left and middle panels show MG-H1-modified proteins detected by Western blot analysis after two-dimensional gel electrophoresis in the HIP of WT/VB6(+) mice and KO/VB6(−) mice, respectively. A typical result from the other two samples providing similar results is presented. HIP samples of WT/VB6(+) mice or KO/VB6(−) mice were subjected to two-dimensional gel electrophoresis followed by CBB staining. The right panel shows a two-dimensional gel image with the CBB staining pattern. The specified spots (spot a, b, c, d, e, and f) were excised and digested in-gel for LC-MS/MS analysis.