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Quantitative detection of MG-H1-modified U-type Creatine kinase by immunoprecipitation. Proteins extracted from the HIP of WT/VB6(+) (A) or KO/VB6(−) (B) mice were immunoprecipitated with mouse IgG or mouse antibody against CK-mit (A,B). HIP homogenate (Input) was loaded as a control. Each sample recovered was electrophoresed by SDS-PAGE (5–20% gradient gel) and immunoblotted with the antibody against MG-H1. A typical result from duplicate experiments is presented.
