Figure 7.

(a,b) Apoptosis detection (AnnexinV/Propidium Iodide) in HEKa cell culture subjected to treatment with UF-OMWW from Italian (a) and Greek (b) OMWWs and UVA irradiation, after 24 h of the treatments (T24), as outlined in Scheme 1. Red and green false colors indicate nuclei stained with PI and cells positive to Annexin V, respectively. (A) Untreated HEKa, (B) HEKa treated with only UVA, (C,E,G) HEKa treated simultaneously with UVA and UF-OMWW, (D,F,H) HEKa treated with UF-OMWW after UVA irradiation. (*) indicates the presence of detached and floating cells. Confocal images are representative of three independent experiments performed in triplicate, and at least 10 fields were kept as confocal images for each sample.