Table 1.

Primers used to detect VDR polymorphisms (26).

Primer sequences	Annealing temperature (°C)	Amplicon size (bp)
FokI (rs10735810)
FokI/F: 5’-TGGCCGCCATTGCCTCCG 3’	62
FokI/f: 5’- TGGCCGCCATTGCCTCCA 3’	60	77
FokI/C: 5’- AGCTGGCCCTGGCACTGA 3’	60
BsmI (rs1544410)
BsmI/B: 5’-AGCCTGAGTACTGGGAATGT-3’	60
BsmI/b:5’- AGCCTGAGTACTGGGAATGC-3’	62	534
BsmI/C:5’- GGGAGGGAGTTAGGCACC-3’	60
TaqI (rs731236)
TaqI/B:5’- CAGGACGCCGCGCTGATT-3’	62
TaqI/b:5’- CAGGACGCCGCGCTGATC-3’5’	60	148
TaqI/C:5’- CCTCATTGAGGCTGCGCAG-3’	62
ApaI (rs7975232)
ApaI/A:5’- TGGGATTGAGCAGTGAGGT-3’	58
ApaI/a:5’- TGGGATTGAGCAGTGAGGG-3’	60	229
ApaI/C:5’- CCTCATTGAGGCTGCGCAG-3’	62
Internal Control
DRBI-F 5’- TGCCAAGTGGAGCACCCAA 3’	60	729
DRBI-R 5’-GCATCTTGCTCTGTGCAGAT 3’	60