Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 20;14:2747–2759. doi: 10.2147/OTT.S293832

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Zhang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

GSEC served as a sponge of miR-202-5p. (A) BT-549 and MDA-MB-231 cells were transfected with miR-202-5p mimics/inhibitor or corresponding negative controls (miR-NC and inhibitor NC). The transfection efficiency was evaluated by qRT-PCR. (B) The putative targeting site between GSEC and miR-202-5p was predicted by Starbase v.2.0. (C) The luciferase reporter activity of Luc-GSEC-WT/MUT was detected by dual luciferase reporter assay. (D) The enrichment of GSEC and miR-202-5p was determined by RIP assay. (E) BT-549 and MDA-MB-231 cells were transfected with sh-GSEC, pc-GSEC (GSEC overexpression), or negative controls (sh-NC and pc-NC). The expression of miR-202-5p was evaluated by qRT-PCR. (F) The expression of miR-202-5p in TNBC tissues and adjacent normal tissues was evaluated by qRT-PCR (n = 45). (G) The expression of miR-202-5p in TNBC tissues of patients at Stage I (n = 11), Stage II (n = 9), Stage III (n = 12) and Stage IV (n = 13) was evaluated by qRT-PCR. (H) The expression of miR-202-5p in TNBC tissues of patients with lymph-node metastasis (n = 15) and non-metastasis (n = 30) was evaluated by qRT-PCR. (I) The correlation between GSEC and miR-202-5p in TNBC tissues was evaluated by Pearson’s correlation analysis (n = 45). ** p < 0.01.