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. 2021 Apr 20;14:2747–2759. doi: 10.2147/OTT.S293832

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© 2021 Zhang et al.

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AXL was a target of miR-202-5p. (A) The putative binding site between miR-202-5p and AXL was predicted by Starbase v2.0. (B) The luciferase reporter activity of Luc-AXL-WT/MUT was detected by dual luciferase reporter assay. (C) BT-549 and MDA-MB-231 cells were transfected with miR-202-5p mimics/inhibitor and corresponding negative controls (miR-NC and inhibitor NC). The expression of AXL was detected by qRT-PCR and Western blot. (D) BT-549 and MDA-MB-231 cells were transfected with pc-GSEC, sh-GSEC, and corresponding negative controls (pc-NC and sh-NC). The expression of AXL was detected by qRT-PCR and Western blot. (E) The expression of AXL in TNBC tissues and adjacent normal tissues was detected by qRT-PCR (n = 45). (F) The correlation between miR-202-5p and AXL in TNBC tissues was evaluated by Pearson’s correlation analysis (n = 45). **p < 0.01.