Skip to main content
. 2021 Apr 20;16:2949–2963. doi: 10.2147/IJN.S304515

Figure 4.

Figure 4

Akk-EVs-treated macrophages suppressed the proliferation and invasion of prostate cells. (A and C) CCK-8 analysis of the viability of human PCa cell line DU145 (A) and PC-3 (C) cells receiving different CM treatments for 24 h. n = 4 per group. (B and D) Representative images and quantitative analysis of the crystal violet-stained colonies formed by DU145 (B) and PC-3 (D) cells receiving different treatments for 14 days. n = 3 per group. (EH) Representative images of scratch wound healing assay in DU145 (E) and PC-3 (G) cells after different CM treatments at indicated time points and corresponding quantification of the migration rates in DU145 (F) and PC-3 (H). Scale bar: 200 μm. n = 4 per group. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001.