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. 2021 Apr 16;2021:6635452. doi: 10.1155/2021/6635452

Figure 4.

Figure 4

Knockdown of TREM1 suppresses the inflammatory response and NF-κB signaling activation in LPS-induced intestinal epithelium cells. (a) Representative images of western blot results for TREM1, p-p65, ad p-IκBa in LPS-induced intestinal epithelium-6 (IEC-6) cells transfected with TREM1 specific siRNA oligos or negative control oligos. (b–d) The expression levels of (b) TREM1 (F value = 26.27), (c) p-p65 (F value = 20.81), and (d) p-IκBa (F value = 72.79) were quantified in the indicated groups. (e–g) The mRNA expression levels of (e) TNF-α (F value = 89.24), (f) IL-6 (F value = 14.15), and (g) IL-1β (F value = 9.290) were evaluated by RT-qPCR. n = 3 for each group. One-way analysis of variance (ANOVA) was used for multiple comparisons, followed by Tukey's test. ns: no statistical significance; ∗∗p < 0.01; ∗∗∗∗p < 0.0001.