Figure 4.

E. koreanum attenuates phosphorylation levels of ERK, Akt (a), Src, and PLCγ2 (b). Immunoblotting was conducted to analyze the phosphorylation of signaling molecules extracted from the lysates of collagen-stimulated washed rat platelets (3 × 10⁸ cells/mL) that were pretreated with vehicle or E. koreanum fraction (EAF). Representative immunoblot images and data (mean ± SD) from at least four independent experiments are shown. Lanes 1–5 of blot images correspond to each bar graph, respectively. ∗p < 0.05 and ∗∗∗p < 0.001 versus the agonist-treated group.