Figure 2.

Effects of EGCG on cell cycle progression of OA-treated QSG-7701 and L02 cells. (a) Flow cytometry assay was used to determine cell cycle distribution. (b) Cell cycle distribution was analyzed. (c) Western blot analysis for the expression levels of cyclin D1, cyclin E1, CDK2, CDK4, p21, and p27 in each group. β-Actin was used as the loading control. (d) The densitometry analysis of each factor was performed in each group, normalized to the corresponding β-actin level. Data are presented as mean ± SEM of three independent experiments; ^∗∗P < 0.01 compared with the control group; ^##P < 0.01 compared with the OA group.