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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Transpl Infect Dis. 2020 Dec 7;23(2):e13514. doi: 10.1111/tid.13514

Figure 2. Clodronate treatment depletes macrophages but not T cells from LDI organs and blood.

Figure 2.

To confirm macrophage depletion by clodronate liposome treatment, RCMV latently-infected donor F344 rats were treated with control liposomes (n=3, black) or clodronate liposomes (n=3, red). At three days post treatment, tissues were harvested and leukocytes were prepared from heart, spleen, and peripheral blood (PBMC). The frequency of CD4+ and CD8+ T cells and macrophages in each of these samples was quantified via flow cytometry by cell surface staining using fluorescent antibodies directed against CD172 (myeloid lineage cells), CD68 (macrophage), CD3 (T cells), CD4 (CD4+ T cells), and CD8 (CD8+ T cells). Depicted is the frequency of macrophages (CD3−CD172+CD68+) and CD4 (CD3+CD4+) and CD8 (CD3+CD8+) cells isolated from the heart (a), spleen (b), and PBMCs (c). **p≤0.01 as determined by Student’s t-test.