Skip to main content
. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Transpl Infect Dis. 2020 Dec 7;23(2):e13514. doi: 10.1111/tid.13514

Figure 3. Clodronate treatment reduces allograft tissue macrophage levels to those observed in uninfected hearts.

Figure 3.

Heart tissue sections from F344 rats that were either uninfected (gray bars), latently RCMV infected and treated with control liposomes (black bars) or latently RCMV infected and treated with clodronate liposome (red bars) were stained with antibodies directed against CD68 (macrophages) (a, d), CD4+ T cells (b) and CD8+ T cells (c). Immunohistochemical analysis was done in a blinded review and scored as follows: 0, no visible staining; 1, faint staining; 2, moderate intensity with multifocal staining; and 3, intense diffuse staining. CD4, CD8 and CD68 positively stained cells with scores 2 and 3 were counted in 10 fields from whole heart tissues at 200x magnification or in 6 fields from the coronary arteries at 400x magnification; and the mean number of positive cells per field was calculated. **p≤0.01 as determined by a One-way ANOVA with Tukey’s secondary testing.