Table 1.
Candidate amiRNA lines with altered response to Arsenite and their target genes
| ID | PHENOTYPE | AMI SEQUENCE | TARGET GENES | GENE DESCRIPTION | VALIDATE D IN T3 | VALIDATED BY RETRANSFORM |
|---|---|---|---|---|---|---|
| 10-9 | More tolerant than WT | TATATAAGAAC CCGAACGCTC | AT5G43350 | Phosphate Transporter 1, PHT1, PHT1;1 | Yes | Yes |
| Root length | AT5G43370 | Phosphate Transporter 2, PHT2, PHT1;2 | ||||
| AT5G43360 | Phosphate Transporter 3, PHT3, PHT1;3 | |||||
| 4-138 | More tolerant than WT | TAACTTCTCAT CCGCACACCG | At2g44940 | Ethylene-response Transcription factor 34 (ERF34) | Yes | Yes |
| At3g60490 | Ethylene-response Transcription factor 35 (ERF35) | |||||
| Root length | At4g25480 | C-Rrepeat/DRE Binding Factor 3 (CBF3), DRE/CRT-Binding Protein 1C (DREB1A), | ||||
| At4g25470 | C-Rrepeat/DRE Binding Factor 2 (CBF2), DRE/CRT-Binding Protein 1b (DREB1B), Freezing Tolerance QTL 4 (FTQ4) | |||||
| At4g25490 | C-Rrepeat/DRE Binding Factor 1 (CBF1), DRE/CRT-Binding Protein 1c (DREB1C) | |||||
| At5g51990 | C-Rrepeat/DRE Binding Factor 4 (CBF4), DRE/CRT-Binding Protein 1d (DREB1D) | |||||
| 4-85 | More tolerant than WT | TATAAGAAATC TTGAGCACCT | AT1G10470 | Response Regulator 4 (ARR4), Induced By Cytokinin 7 (IBC7), Maternal Effect Embryo Arrest 7 (MEE7), | Yes | Yes |
| Root length | AT3G48100 | Response Regulator 5 (ARR5) | ||||
| AT5G62920 | Response Regulator 6 (ARR6) | |||||
| 4-89 | More tolerant than WT | TCTTTCCGAAA GGTCAAACTA | AT1G61660 | basic helix-loop-helix (bHLH) DNA-binding superfamily protein (ATBHLH112) | Yes | n.a |
| Root length | AT3G20640 | basic helix-loop-helix (bHLH) DNA-binding superfamily protein; | ||||
| AT2G31730 | basic helix-loop-helix (bHLH) DNA-binding superfamily protein; | |||||
| 4-82 | More tolerant than WT | TACTTTCCGGT GAGAGTGCGT | AT4G32890 | GATA Transcription Factor 9 (GATA9) | Yes | n.a |
| Root length | AT2G45050 | GATA Transcription Factor 2 (GATA2) | ||||
| AT4G36240 | GATA Transcription Factor 7 (GATA7) | |||||
| 4-184 | More tolerant than WT | ACGGGATTCA AGTCCGAAGCT | AT1G61990 | Mitochondrial Transcription Termination Factor Family Protein | Yes | n.a |
| Root length | AT1G62490 | Mitochondrial Transcription Termination Factor Family Protein | ||||
| AT1G61980 | Mitochondrial Transcription Termination Factor Family Protein | |||||
| AT1G61970 | Mitochondrial Transcription Termination Factor Family Protein | |||||
| AT1G62110 | Mitochondrial Transcription Termination Factor Family Protein | |||||
| AT1G47250 | 20S Proteasome Alpha Subunit 2 (PAF2) |
n.a: not applicable. Phenotypes have not been tested after amiRNA retransformation or via CRISPR or T-DNA gene knock outs.