| Bacterial and Virus Strains |
| Top10 |
Thermo Fisher Scientific |
Cat# 18258012 |
| DH5α |
Thermo Fisher Scientific |
Cat# C404003 |
| DH10Bac |
Thermo Fisher Scientific |
Cat# 10361012 |
| Chemicals, Peptides, and Recombinant Proteins |
| Sodium Butyrate |
Sigma-Aldrich |
Cat# 303410 |
| Lauryl Maltose Neopentyl Glycol |
Anatrace |
Cat# NG310 |
| Digitonin |
Acros Organics |
Cat# 11024-24-1 |
| Anti-DYKDDDDK G1 Affinity Resin |
GenScript |
Cat# L00432 |
| Flag peptide |
Sigma-Aldrich |
Cat# F3290 |
| Antibiotic Antimycotic Solution |
Sigma-Aldrich |
Cat# A5955 |
| Sf-900™ II SFM medium |
Thermo Fisher Scientific |
Cat# 10902088 |
| FreeStyle™ 293 Expression Medium |
Thermo Fisher Scientific |
Cat# 12338018 |
| Critical Commercial Assays |
| Superose 6 Increase 10/300 GL |
GE healthcare |
Cat# 29091596 |
| Deposited Data |
| Coordinates of Apo CNGA1 in K+/Ca2+
|
This paper |
PDB: 7LFT |
| Coordinates of cGMP-bound CNGA1 in K+/Ca2+
|
This paper |
PDB: 7LFW |
| Coordinates of cGMP-bound CNGA1 in Na+
|
This paper |
PDB: 7LFY |
| Coordinates of cGMP-bound CNGA1 in Na+/Ca2+
|
This paper |
PDB: 7LFX |
| Coordinates of cGMP-bound CNGA1_E365Q in Na+/Ca2+
|
This paper |
PDB: 7LG1 |
| Cryo-EM map of Apo CNGA1 in K+/Ca2+
|
This paper |
EMDB: 23306 |
| Cryo-EM map of cGMP-bound CNGA1 in K+/Ca2+
|
This paper |
EMDB: 23307 |
| Cryo-EM map of cGMP-bound CNGA1 in Na+
|
This paper |
EMDB: 23309 |
| Cryo-EM map of cGMP-bound CNGA1 in Na+/Ca2+
|
This paper |
EMDB: 23308 |
| Cryo-EM map of cGMP-bound CNGA1_E365Q in Na+/Ca2+
|
This paper |
EMDB: 23310 |
| Experimental Models: Cell Lines |
| Sf9 cells |
Thermo Fisher Scientific |
Cat# 11496015 |
| HEK293F cells |
Thermo Fisher Scientific |
Cat# R79007
|
| HEK293 cells |
ATCC |
CRL-1573 |
| Oligonucleotides |
| CNGA1_F_primer: gatataGCTAGCaaagataagaaagaagaggagaag |
This paper |
N/A |
| CNGA1_R_primer: gatataGCGGCCGCtcatgtagagtcgatgggcccactttc |
This paper |
N/A |
| Recombinant DNA |
| pEZT-BM vector |
Morales-Perez et al., 2016 |
Plasmid # 74099 (Addgene) |
| pEZT-BM-Flag-CNGA1 and mutations |
This paper |
N/A |
| pNGFP-EU |
Kawate and Gouaux, 2006 |
N/A |
| Software and Algorithms |
| MotionCor2 |
Zheng et al., 2017 |
http://msg.ucsf.edu/em/software/motioncor2.html |
| GCTF |
Zhang, 2016 |
https://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/ |
| RELION |
Scheres, 2012 |
http://www2.mrc-lmb.cam.ac.uk/relion |
| Chimera |
Pettersen et al., 2004 |
https://www.cgl.ucsf.edu/chimera/ |
| Coot |
Emsley et al., 2010 |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot |
| MolProbity |
Chen et al., 2010 |
http://molprobity.biochem.duke.edu/ |
| PyMol |
|
|
| PHENIX |
Adams et al., 2010 |
https://www.phenix-online.org |
| HOLE |
Smart et al., 1996 |
http://www.holeprogram.org/ |
| Others |
|
|
| Quantifoil R1.2/1.3 Au 300 mesh |
Electron Microscopy Sciences |
Cat# Q3100AR1.3 |
