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. Author manuscript; available in PMC: 2022 Apr 21.
Published in final edited form as: Neuron. 2021 Mar 11;109(8):1314–1332.e5. doi: 10.1016/j.neuron.2021.02.023

Figure 6. Synaptotagmin-1 clamps SOCE-driven spontaneous release.

Figure 6.

A. Example traces and 5 s moving averages of frequency of mEPSC recorded from neurons deficient in syt1 (top) or depleted of both STIM2 and syt1 proteins (bottom; same experimental design as in Figure 1A).

B-C. Time courses of average mEPSC frequency normalized to baseline, for hippocampal dissociated neurons from mice (syt1 KD and syt1 KD + STIM2 cKO) from rats (syt1 KD and syt1 KD + BTP2). Insets: Percentage of patched neurons with a significant increase in mEPSC frequency upon TG perfusion (in syt1 KD plus either STIM2 cKO or BTP2).

D-F. Violin plots of baseline frequency (2 mM Ca2+), normalized frequency during TG perfusion and amplitude of mEPSC for mouse (syt1 KD and syt1 KD + STIM2 cKO) and rat (syt1 KD and syt1 KD + BTP2) hippocampal neurons.