KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Guinea pig polyclonal anti-Tau | Synaptic Systems | Cat# 314 004, RRID:AB_1547385 |
| Guinea pig polyclonal anti-MAP2 | Synaptic Systems | Cat# 188 004, RRID:AB_2138181 |
| Mouse monoclonal anti-Synapsin 1 | Synaptic Systems | Cat# 106 011, RRID:AB_2619772 |
| Rabbit polyclonal anti-Synapsin1 | Synaptic Systems | Cat# 106 103, RRID:AB_11042000 |
| Mouse monoclonal anti-PSD95 | Synaptic Systems | Cat# 124 011, RRID:AB_10804286 |
| Mouse monoclonal anti-Rab-GDI1 | Synaptic Systems | Cat# 130 011, RRID:AB_1966443 |
| Rabbit monoclonal anti-GAPDH | Cell Signaling | Cat# 2118, RRID:AB_561053 |
| Rabbit polyclonal anti-Synaptotagmin 7 | Synaptic Systems | Cat# 105 173, RRID:AB_887838 |
| Mouse monoclonal anti-Synaptotagmin 1 | Synaptic Systems | Cat# 105 011, RRID:AB_887832 |
| Mouse monoclonal anti-CHOP (L63F7) | Cell Signaling | Cat# 2895, RRID:AB_2089254 |
| Rabbit polyclonal anti-STIM2 | Cell Signaling | Cat# 4917, RRID:AB_2198021 |
| Rabbit monoclonal anti-STIM1 (D88E10) | Cell Signaling | Cat# 5668, RRID:AB_10828699 |
| anti IRE1α (14C10) - Rabbit monoclonal | Cell Signaling | Cat# 3294, RRID:AB_823545 |
| anti PDI (C81H6) - Rabbit monoclonal | Cell Signaling | Cat# 3501, RRID:AB_2156433 |
| anti GRP78 BiP - Rabbit polyclonal | Abcam | Cat# ab21685, RRID:AB_2119834 |
| anti Basoon - Guinea pig polyclonal | Synaptic Systems | Cat# 141 004, RRID:AB_2290619 |
| anti VGLUT1 - guinea pig antiserum | Synaptic Systems | Cat# 135 304, RRID:AB_887878 |
| anti VGAT - guinea pig antiserum | Synaptic Systems | Cat# 131 004, RRID:AB_887873 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tunicamycin from Streptomyces sp. | Sigma-Aldrich | Catalog # T7765-1MG |
| Thapsigargin | Sigma-Aldrich | Catalog # T9033-5MG |
| BTP2 | Sigma-Aldrich | Catalog # 203890-5MG |
| SKF 96365 | Tocris | Catalog # 1147 |
| D(−)-2-Amino-5-phosphonopentanoic acid (AP-5) | Sigma-Aldrich | Catalog # A8054 |
| Tetrodotoxin (TTX) | Enzo Life Sciences | Catalog # BML-NA120-0001 |
| Picrotoxin (PTX) | Sigma-Aldrich | Catalog # P1675 |
| 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) | Sigma-Aldrich | Catalog # C239-100MG |
| Trypsin from bovine pancreas | Sigma-Aldrich | Catalog # T9935 |
| DNase | Sigma-Aldrich | Catalog # D5025-375KU |
| Matrigel | Corning | Catalog # 354234 |
| FuGENE 6 | Promega | Catalog # E2692 |
| QX-314 | EMD-Millipore | Catalog # 552233 |
| LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells | Thermofisher / Molecular Probes | Catalog # L3224 |
| Ionomycin, free acid | Tocris | Catalog # 1234 |
| A23187, free acid | Tocris | Catalog # 2092 |
| Experimental Models: Cell Lines | ||
| Highly transfectable derivative of human embryonic kidney-293 epithelial adherent cells (HEK293T) | ATCC | Catalog # CRL-1573 |
| Experimental Models: Organisms/Strains | ||
| STIM2 fl/fl C57BL/6 mice pups (P0-1) | Oh-Hora et al., 2008 | N/A |
| Sprague-Dawley rat pups (P2-P4) | Charles River | N/A |
| Recombinant DNA | ||
| Plasmid: pCMV-VSV-G (lentiviral packaging) | Adgene; Stewart et al., 2003 | Catalog # 8454 |
| Plasmid: pRSV-REV (lentiviral packaging) | Adgene; Dull et al., 1998 | Catalog # 12253 |
| Plasmid: pMDLg/pRRE (lentiviral packaging) | Adgene; Dull et al., 1998 | Catalog # 12251 |
| Plasmid: pFUGW-VGluT1-pHGFP | Voglmaier et al., 2006 | N/A |
| Plasmid: pFUGW-Synaptobrevin2-GCaMP6s | Li et al., 2017 | N/A |
| Plasmid: FCK(1.3)GW-ER-GCAM P6-150 | AddGene; de Juan-Sanz et al., 2017 | Plasmid #86918 |
| Plasmid: L309-mCherry-Syt1 KD shRNA | Xu et al., 2012, Bacaj et al., 2013 | N/A |
| Plasmid: L307-Syt7 KD shRNA | Bacaj et al., 2013 | N/A |
| Plasmid: pFUGW-Syt7 WT (shRNA resistant) | Li et al., 2017 | N/A |
| Plasmid: pFUGW-Syt7 5DA (shRNA resistant) | Bacaj et al., 2015 | N/A |
| Plasmid: pFUGW-Syt7 MK | Voleti et al., 2017 | N/A |
| Plasmid: pFUGW-GFP-Cre | Sequence from Lin et al., 2018 | N/A |
| Plasmid: human STIM1 in pDS_XB-YFP vector | Brandman et al., 2007 | N/A |
| Plasmid: human STIM2 in pDS_XB-YFP vector | Brandman et al., 2007 | N/A |
| Rat STIM1 shRNA 1 | GCATGGAAGGCATCAGAAGTGTATAACTG | |
| Rat STIM1 shRNA 2 | GTCCCTGGATGACGTGGATCATAAAATCT | |
| Rat STIM2 shRNA 1 | GGCTGAAAAGGAGTTTGAACTGAGAAGCA | |
| Rat STIM2 shRNA 2 | GCCAGAAGCAGTAGTTTATGCCGCTCTCG | |
| Scramble shRNA control | GCACTACCAGAGCTAACTCAGATAGTACT | |
| Software and Algorithms | ||
| Matlab script for pHluorin analysis | Chanaday et al., 2018a | N/A |
| Fiji | Schindelin et al., 2012 | N/A |
| GelAnalyzer 2010a | http://www.gelanalyzer.com/ | N/A |