Figure 2.
GBP1 microcapsules around S. flexneri interfere with septin caging. HeLaGBP1-KO cells stably expressing mCherry-GBP1 were infected with S. flexneri ΔipaH9.8, fixed after 2.5 hpi, and immunostained for endogenous Sept7. Co-localization of GBP1 and Sept7 on bacteria was quantified. Mean frequencies ± SEM of combined data from three independent experiments are shown. Significance was determined by unpaired two-tailed t-test. *P ≤ 0.05. Arrow heads point at septin cages. All scale bars equal 5 µm.