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. 2021 Apr 10;10(4):867. doi: 10.3390/cells10040867

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Contribution of hTERT downregulation to functional impairment of migration and adhesion in MCF7 and MDA-MB-231 cells. (A) Adhesion assay. The photos are representative of three independent experiments (magnification 40×). Adhesion cells were counted in at least three fields in each well. Each bar represents the mean ± SD of the data obtained from three independent experiments. (*) p < 0.05 relative to shRNA Control. (B) Wound-healing migration assay. Cells were scraped with the pipette tip. The photos represent cell migration under the microscope at 100× magnification field after the injury. A typical result out of three replicates was demonstrated. The migration of studied cells was quantified by measuring wound closure areas after injury. Each bar represents mean ± SD (n = 3). (*) p < 0.05 relative to shRNA Control cells (C) immunodetection of β1-integrin, FAK, p-FAK (Tyr397), p-FAK (Tyr576/577), paxillin, p-paxillin, Src, p-Src (Tyr527), and p-Src (Tyr416) was performed 21st-day post-transduction, using Western blot; 0.1 μM DOX; 8 h treatment, followed by densitometry analysis. (*) p < 0.05; (**) p < 0.01 relative to shRNA Control. Densitometry analysis was performed on three scanned membranes from three independent experiments.

Contribution of hTERT downregulation to functional impairment of migration and adhesion in MCF7 and MDA-MB-231 cells. (A) Adhesion assay. The photos are representative of three independent experiments (magnification 40×). Adhesion cells were counted in at least three fields in each well. Each bar represents the mean ± SD of the data obtained from three independent experiments. (*) p < 0.05 relative to shRNA Control. (B) Wound-healing migration assay. Cells were scraped with the pipette tip. The photos represent cell migration under the microscope at 100× magnification field after the injury. A typical result out of three replicates was demonstrated. The migration of studied cells was quantified by measuring wound closure areas after injury. Each bar represents mean ± SD (n = 3). (*) p < 0.05 relative to shRNA Control cells (C) immunodetection of β1-integrin, FAK, p-FAK (Tyr397), p-FAK (Tyr576/577), paxillin, p-paxillin, Src, p-Src (Tyr527), and p-Src (Tyr416) was performed 21st-day post-transduction, using Western blot; 0.1 μM DOX; 8 h treatment, followed by densitometry analysis. (*) p < 0.05; (**) p < 0.01 relative to shRNA Control. Densitometry analysis was performed on three scanned membranes from three independent experiments.