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. 2021 Apr 12;10(4):870. doi: 10.3390/cells10040870

Figure 2.

Figure 2

In vitro generated CII-specific ACAID B cells inhibited CII-induced DTH responses in C57BL/6 mice. The in vitro generation of CII-specific ACAID B cells and the DTH assay were described in the Materials and Methods section. CII-specific ACAID B cells were intravenously injected to induce ACAID followed by subcutaneous immunization with CII/CFA on day 7. On day 14, mice were challenged with CII (500 μg in 20 μL) intradermally in the left ear pinna, and 20 μL of acetic acid alone was injected into the right ear pinna as an internal control. ACAID induction was confirmed by suppression of ear swelling responses after 24 h (left panel) and 48 h (right panel). The positive control mice were subcutaneously immunized with CII/CFA on day 7 and with CII on day 14, whereas the negative control mice only received intradermal injection of CII on day 14. * p < 0.05 were considered to be statistically significant.